Application
Useful for kinase assay and Western blot.
Biochem/physiol Actions
Recombinant full-length human MEK5 was expressed by Baculovirus in Sf9 insect cells using an N-terminal GST tag. MEK5 or MAP2K5 (mitogen-activated protein kinase kinase 5) is a dual specificity protein kinase that belongs to the MAP kinase kinase family which specifically interacts with and activates MAPK7/ERK5. MEK5 itself can be phosphorylated and activated by MAP3K3/MEKK3, as well as by atypical protein kinase C isoforms (aPKCs). The signal cascade mediated by MEK5 is involved in growth factor stimulated cell proliferation and muscle cell differentiation (1). The rs3743354 polymorphism in the MEK5 promoter may affect the risk of developing colorectal cancer (CRC) (2).
Legal Information
PRECISIO is a registered trademark of Sigma-Aldrich Co. LLC
Reconstitution
Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.
Storage and Stability
Store in aliquots at -70°C. Avoid freeze/thaw cycles.
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